Vnitr Lek 1993, 39(10):954-958

[Identification of HLA-D allele regions using DNA analysis].

M Loudová, R Brdicka, M Láblerová, P Korínková, P Iványi: Ustav hematologie a krevní transfuze, Praha.

Klíčová slova: Alleles; DNA Fingerprinting; HLA-D Antigens, genetics, ; Histocompatibility Testing, methods, ; Humans; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length

The successful bone marrow transplantation presents the highest demands on the compatibility between donor and recipient in the antigen products of HLA system, namely in transplantations performed in unrelated individuals. Traditionally used serotyping gives rather exact results in class I HLA antigen determination, but when typing of class II antigens, at least 25% errors occur. Our papers presents a comparison of different molecular genetics methods used in class II HLA antigen typing. First results of class II HLA typing using RFLP technique, as performed in the Institute of Hematology and Blood Transfusion, are summarized.

Keywords: Alleles; DNA Fingerprinting; HLA-D Antigens /genetics/; Histocompatibility Testing /methods/; Humans; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length

Zveřejněno: 1. říjen 1993 Zobrazit citaci

Loudová M, Brdicka R, Láblerová M, Korínková P, Iványi P. [Identification of HLA-D allele regions using DNA analysis]. Vnitr Lek. 1993;39(10):954-958.

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